I wish to know the role of biotin in BMGY (Buffered Glycerol Complex Medium) Other articles mostly add it depending on the dissolved oxygen or methanol in the medium, but we do not have way to

Buffered Glycerol Complex Medium, Sterile 2.0% Peptone, 1.0% Yeast extract, 1.34% Potassiumphosphate pH 6.0, 1.34% Yeast nitrogen base w/o AA, 0.4 mg/L Biotin, 1.0% Feb 27, 2017 · Buffered glycerol-complex medium. CD: Circular dichroism. HPLC: High performance liquid chromatography. pNPR: p-Nitrophenyl α-L-rhamnopyranoside. YNB: Yeast nitrogen base. YPD: Yeast extract peptone dextrose medium Applications of the GST- Affinity Tag in the Purification and Characterization of Proteins A dissertation submitted in partial fulfillment of the requirements for the degree of May 23, 2017 · The transformants were selected on YPD (yeast extract peptone dextrose) plates containing 200 μg/mL zeocin. The selected clones were inoculated and amplified in 30 mL of buffered glycerol-complex medium at 30 °C for 2 days. Then the culture medium was replaced by 20 mL of buffered methanol-complex medium to induce protein expression. Twenty transformants were used to inoculate 40 ml of buffered glycerol complex medium (BMGY) (Invitrogen) (0.1 M potassium phosphate buffer at pH 6.0 containing 1% [wt/vol] yeast extract, 2% [wt/vol] peptone, 1.34% [wt/vol] yeast nitrogen base without amino acids, 1% [vol/vol] glycerol, and 4 × 10 −5 % [wt/vol] biotin). Cultures were grown Then, the culture was transferred into 1 L fresh BMGY, and all of the cells were collected by centrifugation at room temperature (3,500 g for 5 min) until the of the culture reached approximately 6, at which point it was resuspended in 100 mL of buffered methanol-complex medium (BMMY). Methanol (3%) was added to the media daily to induce the Jul 27, 2017 · The cells were then recovered by adding 500 µL liquid LB medium and incubating at 37 °C for 1 h and then plated on LB plates supplemented with 25 μg/mL Zeocin (TFS, R25001). After incubated at 37 °C overnight, ten colonies were cultured in 3 mL liquid LB medium supplemented with 25 µg/mL Zeocin at 37 °C overnight.

Twenty transformants were used to inoculate 40 ml of buffered glycerol complex medium (BMGY) (Invitrogen) (0.1 M potassium phosphate buffer at pH 6.0 containing 1% [wt/vol] yeast extract, 2% [wt/vol] peptone, 1.34% [wt/vol] yeast nitrogen base without amino acids, 1% [vol/vol] glycerol, and 4 × 10 −5 % [wt/vol] biotin). Cultures were grown

Buffered Glycerol Complex Medium, Sterile 2.0% Peptone, 1.0% Yeast extract, 1.34% Potassiumphosphate pH 6.0, 1.34% Yeast nitrogen base w/o AA, 0.4 mg/L Biotin, 1.0%

Buffered Glycerol Complex Medium, Sterile 2.0% Peptone, 1.0% Yeast extract, 1.34% Potassiumphosphate pH 6.0, 1.34% Yeast nitrogen base w/o AA, 0.4 mg/L Biotin, 1.0%

Dec 27, 2018 · Buffered dextrose-complex medium. BMGY: Buffered glycerol-complex medium. BMMY: Buffered methanol-complex medium. BMNY: Buffered complex medium without a carbon source. GFP: green Fluorescent protein. KM71m: Mxr1-reprogrammed KM71 strains. Mxr1: Methanol expression regulator 1. P AOX1. Alcohol oxidase 1 promoter. scFv: Single-chain variable Inoculates for the batch cultures were grown on a buffered glycerol-complex medium (BMGY; 10 g/L yeast extract, 20 g/L peptone, 100 mmol/L potassium phosphate, pH 6.0, 13.4 g/L YNB, 4×10-4 g/L biotin, 10 g/L glycerol). Vogel's Medium N has become the medium of choice when a defined synthetic medium is needed for such applications as routine auxanography, stock-keeping, searching for auxotrophic mutants, or growing Neurospora to prepare DNA, mitochondria, etc. However, another medium ("Bird Medium", Metzenberg 2004) has been designed CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Abstract- The Aspergillus ficuum phytase gene phyA was overexpressed in Pichia pastoris X33 after replacing Buffered glycerol-complex medium (BMGY medium) using 1 % (v/v) glycerol with fresh Buffered methanol-complex medium (BMMY medium) using 1 % (v/v) methanol (on daily basis) as carbon sources.